11/14/2022 0 Comments Fhdb 1.95 downloadNeem leaf extract as potential modifier of free radical interaction with RBC membrane verticilloides metabolite was extracted using dichloromethane and phosphoric acid (10:1) while powdered neem leaf was extracted with ethanol for 72 h. from the seeds samples were Aspergillus spp. The experiment, which .Įffects of Fusarium verticilloides, its metabolites and neem leaf. flavus of neem leaves between extracts in polar and nonpolar solvents.Įffects of Fusarium verticilloides, its metabolites and neem leaf. CONCLUSION There is no significant difference in in-vitro inhibitory effectivity on A. At higher concentrations, the antifungal effect of NLHE is better than that of NLEE. RESULTS NLHE had a higher, but statistically non-significant, inhibitory effect on A. Statistical analysis used Chi square and Fisher’s exact test. Fungal growth was detected on Sabouroud dextrose agar. These comprised the neem leaf ethanol extract (NLEE at 0.5, 1.0, and 2.0 g/dL, neem leaf hexane extract (NLHE at 0.5, 1.0, and 2.0 g/dL, positive control, and negative control groups. A macrodilution test with 48-hour incubation time was done in triplicate on 8 groups of samples. Neem leaves were extracted in ethanol or hexane at various concentrations. METHODS An in vitro experimental research was conducted between October 2013 and January 2014. The purpose of this research was to compare the antifungal effect of neem leaves extracted in a nonpolar solvent to that of leaves extracted in a polar solvent. However, most chemical compounds with antifungal effect are nonpolar. Previously we found that the ethanol extract of neem (Azadirachta indica A Juss leaves inhibits A. Medicinal plants are increasingly used as they are relatively safer with minimal side effects. Hexane neem leaf extract more potent than ethanol extract against Aspergillus flavusĭirectory of Open Access Journals (Sweden)įull Text Available BACKGROUND Aspergillus flavus is one of the causes of aspergillosis, with a high virulence and resistance to standard antifungals, resulting in a high mortality rate.
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